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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Biomaterials. 2017 Dec 26;159:130–145. doi: 10.1016/j.biomaterials.2017.12.019

Fig. 4. Chlamydia-specific intracellular cytokine production from CD3+CD4+ IFN-γ secreting T cells.

Fig. 4

Fig. 4

DCs were primed with PPM, M278 or PPP before co-culturing with T cells from naïve and C. muridarum vaccinated mice. For intracellular IFN-γ secretion, cells were stained with CD3-APC-Cy7 and CD4-PerCP-Cy5.5 followed by staining with IFN-γ-APC. Analyses were performed by gating on CD3+ IFN-γ secreting cells for naïve ((A, B, C) and vaccinated co-cultures (D, E, F) and also for CD4+ IFN-γ secreting cells from naïve (G, H, I) and vaccinated (J, K, L) mice.