CSFE-labeled T cells from naïve (A, B, C) and vaccinated (D, E, F) mice were co-cultured with primed DCs and analyzed by flow cytometry for CD3+ CFSE+ T cells. Primed DCs were co-cultured with T cells from naïve and vaccinated mice and induction of memory, and effector T cell phenotypes were quantified by staining for CD3, CD4, CD62L, and CD44, respectively. Analyses were performed by gating on CD3+ CD4+ T cells of naïve (G, H, I) and vaccinated (J, K, L) co-cultures.