DCs were stimulated for 24 hours with PPM, M278 and PPP followed by staining for the subcellular organelles EE (EEA1, early endosome) (A), LE (Rab7, late endosome) (B), ER (endoplasmic reticulum) (C) and lysosome (LAMP-1) (D). Colocalization with organelles was confirmed by probing for the expression of the targeted M278. DAPI (blue) was used to stain the nuclei. The top row (merge) indicate the overlay of images. Direct visualization and imaging were performed employing immunofluorescence microscopy.