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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Mol Cell Cardiol. 2017 Nov 21;114:220–233. doi: 10.1016/j.yjmcc.2017.11.014

Figure 6. SERCA2a free cysteine labeling is altered by HAX-1.

Figure 6

Homogenates from WT and HAXiKO hearts were treated with biotin maleimide to quantify labeling of free cysteines. Biotin and SERCA2a levels were simultaneously assessed by blotting with fluorescent streptavidin and SERCA2a polyclonal Ab/fluorescent secondary Ab after SDS-PAGE. (A) Representative blots for biotin, SERCA2a, and overlay; (B) corresponding quantification. Biotin and PLN levels were simultaneously assessed by blotting with fluorescent streptavidin and PLN antibody. (C) Representative blots for biotin, PLN, and overlay, and (D) corresponding quantification. Data are presented as mean ± SEM (P < 0.05: * vs WT).