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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Biochemistry. 2018 Jan 8;57(5):540–546. doi: 10.1021/acs.biochem.7b00938

Figure 2.

Figure 2

RT in-cell NMR maintains E.coli and HeLa cells in a physiologically stable state for up to 24 hours. A. Signal intensity of cellular 31P-containing metabolites, including ATP, ADP, NAD+ and NAD(H), over time for HeLa cells with (square) and without (circle) flow of DMEM. B. Signal intensity of cellular 31P-containing metabolites over time for E.coli cells with (square) and without (circle) flow of minimal medium. Error bars are the standard deviation of each data point. C. HeLa cells are 99 ± 1% viable immediately after casting. D. HeLa cells are 99 ± 1% viable 24 h after casting; the increase in cell density is consistent with on-going metabolic activity. White dashed lines outline the threads. HeLa cell samples contained 8 × 107 cells. E. coli samples contained cells from 100 mL of culture suspended in ~0.7 mL.