Table 2.

Interpretation of results and result summary of known negative cattle sera using the LFA kit

S. no	Fusion protein	BoPPD	AvPPD	Interpretation	Number of reactive sera/total TB negative cattle sera	Conclusion based on the LFA kit
1	Positive line	Positive line	Positive line	TB sero-positive	–	TB sero-positive (10 out of 100 samples)
2	Positive line	Positive line	Negative line		1 out 100
3	Negative line	Positive line	Negative line		3 out 100
4	Positive line	Negative line	Negative line		3 out 100
5	Positive line	Negative line	Positive line		2 out 100
6	Negative line	Positive line	Positive line	TB sero-positive/Inconclusive	1 out 100
7	Negative line	Negative line	Positive line	TB sero-negative (positive for environmental mycobacteria)	6 out of 100	TB sero-negative (90 out 100 samples)
8	Negative line	Negative line	Negative line	TB sero-negative	84 out of 100

The sera samples (n = 100) were from cattle which turned negative in skin test and IGRA. The estimated specificity of the LFA kit was 90% (82.38–95.10% at 95% CI) in cattle