Table 1.
Disc diffusion assay of methanol extract and its solvent-soluble fractions from Eisenia bicyclis against Listeria monocytogenes
| Strains | Conc. (mg/disc) | Zone of inhibition (mm)a | |||||
|---|---|---|---|---|---|---|---|
| MeOHb | Hexane | DCM | EtOAc | BuOH | H2O | ||
| KCTC 19112 | 1 | 9.5 ± 0.2c | 8.0 ± 0.1 | 8.0 ± 0.3 | 9.5 ± 0.4 | ND | ND |
| 5 | 13.0 ± 0.5 | 10.0 ± 0.4 | 10.0 ± 0.5 | 15.0 ± 0.1 | 9.0 ± 0.2 | ND | |
| Strain 2148 | 1 | 10.0 ± 0.2 | 7.5 ± 0.2 | 7.0 ± 0.3 | 10.0 ± 0.4 | ND | ND |
| 5 | 14.0 ± 0.1 | 9.0 ± 0.1 | 8.5 ± 0.4 | 14.5 ± 0.3 | 10.0 ± 0.1 | ND | |
| Strain 2637 | 1 | 9.0 ± 0.5 | 7.5 ± 0.4 | 7.5 ± 0.5 | 9.5 ± 0.4 | ND | ND |
| 5 | 13.0 ± 0.1 | 8.5 ± 0.1 | 10.0 ± 0.1 | 14.0 ± 0.2 | 9.5 ± 0.2 | ND | |
| Strain 2868 | 1 | 9.0 ± 0.2 | ND | ND | 9.0 ± 0.2 | ND | ND |
| 5 | 12.0 ± 0.5 | 8.0 ± 0.1 | 8.5 ± 0.4 | 13.5 ± 0.2 | 8.0 ± 0.3 | ND | |
ND no detected antibacterial activity
a Methanol extract and its fractions from E. bicyclis were loaded onto discs (6 mm in diameter)
b MeOH methanolic extract, Hexane n-hexane-soluble fraction, DCM dichloromethane-soluble fraction, EtOAc ethyl acetate-soluble fraction, BuOH n-butanol-soluble fraction, H 2 O water-soluble fraction
c Data are the averages of triplicate experiments