Table 2. Prevalence of Cryptosporidium species and genotypes in domestic animals including pets and wild animals using RFLP or/and sequencing of PCR products, Czech Republic, 2003–2016.
| Animals | n positive | n total | % (95% CI) | Cryptosporidium spp. | Cryptosporidium subtypes | Method (and sequenced molecular markers) | Reference |
|---|---|---|---|---|---|---|---|
| Domestic | |||||||
| Cattle | 11a | NA | NA | C. parvum, C. andersoni | NA | PCR (SSU rRNA, HSP70) | [122] |
| 44 | 995 | 4.42 (3.27–5.84) | C. andersoni, C. parvum, C. bovis | IIaA15G2R1 | PCR (SSU rRNA, gp60) | [123] | |
| 56 | 309 | 18.12 (4.12–22.72) | C. andersoni, C. parvum, C. bovis | IIaA16G1R1, IIaA22G1R1, IIaA18G1R1, IIaA15G1R1 | PCR (SSU rRNA, gp60) | [119] | |
| Pigs | 1a | NA | NA | C. suis | NA | PCR (SSU rRNA, HSP70) | [122] |
| 34 | 123 | 27.64 (20.29–36.04) | C. suis, C. scrofarum, C. parvum | IIaA16G1R1 | PCR (SSU rRNA, gp60) | [124] | |
| 69 | 413 | 16.71 (13.34–20.54) | C. suis, C. scrofarum, C. muris | NA | RFLP (SSU rRNA) | [125] | |
| 177 | 477 | 37.11 (32.86–41.51) | C. suis, C. scrofarum | NA | RFLP (SSU rRNA) | [126] | |
| 353 | 1620 | 20.79 (7.95–16.67) | C. suis, C. scrofarum, C. parvum, C. muris | NA | RFLP (SSU rRNA) | [127] | |
| Horses | 12 | 352 | 3.41 ( 1.86–5.72) | C. muris, horse genotype, C. parvum, C. tyzzeri | IVaA15G4, IIaA15G2R1, IXbA22R9 | PCR (SSU rRNA, gp60) | [128] |
| Cat | 1a | NA | NA | C. felis | NA | PCR (SSU rRNA, HSP70) | [122] |
| Red-crowned parakeets | 4a | NA | NA | C. avium | NA | PCR (SSU rRNA, HSP70, actin) | [129] |
| Tortoises | 46 | 387 | 12.66 (8.66–15.11) | C. testudinis, C. ducismarci, tortoise genotype III | NA | PCR (SSU rRNA, COWP, actin) | [42] |
| Wild | |||||||
| Wild boars | 32 | 193 | 16.58 (11.83–22.33) | C. suis, C. scrofarum | NA | RFLP (SSU rRNA) | [130] |
| 61 | 460 | 13.26 (10.39–16.60) | C. suis, C. scrofarum | NA | RFLP (SSU rRNA) | [131] | |
| Giraffe | 1a | NA | NA | C. muris | NA | PCR (SSU rRNA) | [132] |
| Ungulates | 6a | NA | NA | C. ubiquitum, C parvum, C. andersoni | NA | PCR (SSU rRNA, HSP70) | [122] |
| 10 | 269 | 3.72 (1.90–6.52) | C. ubiquitum, C. muris, deer genotype | XIId | PCR (SSU rRNA, gp60) | [133] | |
| Birds | 17a | NA | NA | C. meleagridis, C. baileyi | NA | PCR (SSU rRNA, HSP70) | [122] |
| 663b | NA | NA | C. baileyi, C. meleagridis | NA | PCR (HSP70) | [134] | |
| 85b | NA | NA | C. baileyi, C. meleagridis | IIIeA16G2R1c | PCR (SSU rRNA, HSP70, gp60) | [135] | |
| Mouse | 14a | NA | NA | C. tyzzeri | IXaA6, IXaA8, IXbA6, | PCR (SSU rRNA, gp60, actin, COWP, TRAP-C1) | [136] |
| Rodents | 7 a | NA | NA | C. muris, C. andersoni | NA | PCR (SSU rRNA, HSP70) | [122] |
| Siberian chipmunks | 1 | 1 | 100 (5–100) | C. muris | NA | PCR (SSU rRNA) | [137] |
| Reptiles | 10a | NA | NA | C. serpentis, C. varanii, C. muris | NA | PCR (SSU rRNA, HSP70) | [122] |
| Rabbits | 2 | 2 | 100 (22.36–100) | C. cuniculus | NA | PCR (SSU rRNA, HSP70) | [122] |
| Hedgehogs | 12 | 15 | 80 (54.65–94.65) | C. parvum, C. erinacei | NA | PCR (SSU rRNA, gp60) | [138] |
| Bats | 3 | 263 | 1.14 (0.29–3.07) | C. parvum, bat genotype III | NA | PCR (SSU rRNA) | [139] |
CI: confidence interval; COWP: Cryptosporidium oocyst wall protein; gp60: 60 kilodalton glycoprotein; HSP70: 70 kilodalton heat shock protein; NA: not available; RFLP: restriction fragment length polymorphism; SSU rRNA: small subunit ribosomal ribonucleic acid; TRAP-C1: thrombospondin related adhesive proteins.
a Selected samples.
b Pooled samples.