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Decreased expression of IGF-1 in hypoxic HTR8 and JEG3 cells. A, D. ELISA showed decreased supernatants IGF-1 levels in hypoxic HTR8 and JEG3 cells, respectively. B, E. Western blot revealed reduced IGF-1 protein levels in hypoxic HTR8 and JEG3 cells, respectively. C, F. qRT-PCR depicted a reduction of IGF-1 mRNA levels in hypoxic HTR8 and JEG3 cells, respectively. Data were presented as Mean ± SD. *P<0.05, **P<0.01, ***P<0.001.
