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. 2018 Jan 15;10(1):16–39.

Figure 9.

Figure 9

IGF-1 was upregulated caused by its hypomethylated promoter region in HTR8 cells treated with 5-Aza-dc and DNMT1-siRNA. A, B. Increased IGF-1 in HTR8 cells treated with 4µM 5-Aza-dc. C, D. Increased IGF-1 in HTR8 cells treated with DNMT1-siRNA. E. Representative image of DNA integrity verified by agarose gel electrophoresis. F. IGF-1 promoter region was hypomethylated at CpG_1 and CpG_2 in HTR8 cells treated with 5-Aza-dc and DNMT1-siRNA. G. Representative pyrosequencing images of IGF-1 in four groups. Data were shown as Mean ± SD. *P<0.05, **P<0.01, ***P<0.001. NC refers to negative control.