Table 2.

Optimal dilutions Primary antibodies for WB

Placentas			Trophoblasts
Antibodies	Dilutions	Sources, Cat. No.	Antibodies	Dilutions	Sources, Cat. No.
IGF-1	1:500	Abcam, USA, ab9572	IGF-1	1:2000	Abcam, USA, ab9572
DNMT1	1:1000	Abcam, USA, ab19905	DNMT1	1:1000	Abcam, USA, ab13537
DNMT3a	1:1000	Abcam, USA, ab4897	DNMT3a	1:2000	Abcam, USA, ab13888
DNMT3b	1:500	Abcam, USA, ab119282	DNMT3b	1:2000	Abcam, USA, ab13604
TET1	1:500	Abcam, USA, ab191698	TET1	1:1000	Abcam, USA, ab105475
TET2	1:1000	Abcam, USA, ab94580	TET2	1:1000	Abcam, USA, ab125084
TET3	1:500	Novus, USA, NBP2-20602	TET3	1:1000	Abcam, USA, ab139805
β-actin	1:1000	Beyotime, China, AA128	GAPDH	1:1000	CST, USA, #2118