Multi-differentiation of UCMSCs in vitro. A. Undifferentiated UCMSCs displayed fibroblast-like cell morphology. B. Von Kossa staining for osteogenic differentiation. C. Oil red O staining for adipogenic differentiation. D. Neurofilament M immunofluorescence staining for neurogenic differentiation. E-P. Immunophenotypic characterization of UCMSCs by flow cytometry. The cells were positive for CD49, CD90, CD29, CD271, CD73, CD105, CD166, and CD44 but negative for CD34, CD45, CD123, and HLA-DR.