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. 2018 Jan 15;10(1):224–234.

Figure 2.

Figure 2

miR-132 directly inhibits the expression of USP9X via binding its 3’UTR. A: The predicted miR-132 binding site in the 3’UTR of USP9X by microRNA database. B: mRNA expression of USP9X in A549 and NCI-H1299 cells by quantitative RT-PCR. * P < 0.05 vs A549. C: Protein expression of USP9X in A549 and NCIH-1299 cells determined by western blot. D: Relative expression of miR-132 in A549 and NCI-H1299 cells by quantitative RT-PCR. ** P < 0.01 vs A549. E, F: mRNA expression of USP9X in cells transfected withnegative control siRNA, or miR-132 mimic, or miR-132 inhibitor by quantitative RT-PCR. The data in each group was normalized to the control (* P < 0.05) or miR-132 mimic (# P < 0.05). G: Protein expression of USP9X in cells transfected with negative control siRNA, or miR-132 mimic, or miR-132 inhibitor determined by western blot.