Effect of c-KIT on GIST cell viability, proliferation, and apoptosis in vitro. A, B. CCK-8 assay of the viability of GIST cells treated with c-KIT siRNA, Negative siRNA, or Control in the presence of imatinib (0, 20, 40, 60, 80 μM). C, D. EdU assay of cell proliferation rate under IC50 of imatinib in GIST cells treated with c-KIT siRNA or control siRNA. The number of EDU-positive cells was counted. E. Flow cytometry analysis of apoptosis in GIST cells transfected with control siRNA or CCDC26 siRNA (*P<0.05).