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. 2018 Feb 2;9:150. doi: 10.3389/fimmu.2018.00150

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Copyright © 2018 Lieberman, DeGolier, Haberthur, Chinn, Moyes, Bouchlaka, Walker, Capitini and Crane.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription factor expression changes following expansion. (A) Tbet mRNA expression in total natural killer (NK) cells assessed by nanostring (NS) at day 0 or day 12. Statistical significance was assessed by Student’s t-test. (B) Eomes mRNA expression in total NK cells assessed by NS at day 0 or day 12. (C) Fold change by qPCR calculated by the ΔΔCt method for Tbet and Eomes. Statistical significance was assessed by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). (D) Ratio of the fold change for Eomes relative to the fold change in Tbet as calculated for NS and qPCR.