FIGURE 10.

Expression analysis of key genes involved in iron and heme metabolism in the insect midgut compartments. R. prolixus adult females were fed on blood and the anterior and PMs were dissected immediately before (day 0) or at the indicated times after the blood-meal for qPCR analysis. Heme oxigenase (A), Ferritin (B), Iron Responsive Protein 1 (IRP1) (C), and Feline Leukemia Virus subgroup C Receptor (FLVCR) (D). The elongation factor 1 gene (RPRC007684) was used as an endogenous control (n = 12–15). Data shown in all graphics are mean ± SE. One-way ANOVA followed by Tukey’s multiple comparison test was used to evaluate differences in gene expression during the days after blood ingestion. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.05, ^∗∗∗∗p < 0.0001. These data are resulted from three independent experiments.