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. 2018 Feb 2;9:19. doi: 10.3389/fgene.2018.00019

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Copyright © 2018 Walter-Nuno, Taracena, Mesquita, Oliveira and Paiva-Silva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of gene silencing in the production of ROS in the midgut of adult insects. Starved adult females were injected with 1 μg of dsRNA of ferritin or heme oxygenase (HO). After 48 h, the animals were fed on blood. Dissected posterior midguts were incubated at 28°C for 30 min in a solution containing 100 μM DHE. dsMal-injected females were used as a control. The tissues were analyzed by Fluorescence Microscopy (Zeiss Axio Observer Microscope) using a 20× lens with 80 ms exposure time for all conditions. (A) The images are representative of a total of 12 intestines analyzed. (B) Quantification of fluorescence using Zeiss Axio Observer Quantification software. P = 0.0016. One-way ANOVA with Tukey post test. ^∗∗∗∗p < 0.0001 scale bar 2 μm.