Figure 1.

Pristane increases HIF1α, TNFα, and glycolysis. B6 mice were injected i.p. with pristane and MO. Peritoneal cells were collected at d14 and RNA was extracted. (A) Expression of Hif1a and Tnfa mRNA relative to 18 S (Q-PCR). *P < 0.05 vs. control (unpaired Welch’s t-test). (B) Peritoneal CD11b⁺Ly6C^high cells were flow-sorted from pristane- and MO-treated mice, and Hif1a expression was measured by Q-PCR. *P = 0.05 vs. control (unpaired Welch’s t-test). (C) Extracellular flux analysis of adherent peritoneal cells from pristane- and MO-treated mice (14 days after treatment). After 1 h incubation, oxygen consumption rate (OCR) was determined with sequential addition of 1 µg/ml oligomycin (Oligo), 400 nM FCCP, and 1 µM rotenone + 1 µM antimycin A (Rot + Ant). (D) Effects of pristane and MO on basal OCR (left) and extracellular acidification rate (ECAR, right) (XF96 Analyzer). Experimental treatments were performed with five technical replicates and three biological replicates. *P < 0.05 vs. control (unpaired Student’s t-test). (E) Intracellular TNFα staining of CD11b⁺Ly6C^high cells from pristane vs. MO treated mice. *P < 0.05 vs. control (unpaired Student’s t-test).