Figure 2.

Pristane decreases LXRα activity in PEC. B6 mice were injected i.p. with pristane or MO. PEC were collected at d14 and RNA was isolated. (A) Q-PCR for Nr1h3 and Abca1 expression relative to 18 S. **P < 0.01, Welch’s t-test. (B) PEC from wild-type mice were stimulated with 1 µM GW3965 for 24 h. Nr1h3 and Abca1 expression levels were determined by Q-PCR (representative of three experiments). (C) CD138⁺CD11b⁺ cells from pristane- and MO-treated mice were flow sorted, and mRNA was analyzed by Q-PCR. Left, Abca1; right, Nr1h3. **P < 0.05, Student’s t-test (left) and Welch’s t-test (right). (D) Peritoneal CD11b⁺Ly6C^hi and CD11b⁺CD138⁺ cells from MO-treated mice were flow sorted, and Abca1 expression was analyzed (Q-PCR). *P < 0.05 (paired Student’s t-test). (E) Peritoneal cells from pristane- and MO-treated mice were stained with antibodies against CD11b, CD138, Ly6C, and Abca1. Mean Fluorescence Intensity (MFI) of Abca1 staining (flow cytometry) was compared between CD11b⁺Ly6C^hi and CD11b⁺CD138⁺ subsets. **P < 0.01; ***P < 0.001 vs. control (paired Student’s t-test).