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. 2018 Feb 2;9:135. doi: 10.3389/fimmu.2018.00135

Figure 4.

Figure 4

Inverse relationship of LXRα and HIF-1α. (A) Peritoneal Ly6Chi and CD138+ Mϕ were flow sorted from pristane-treated mice, and Hif1a mRNA expression was measured relative to 18 S (Q-PCR). **P < 0.01, Student’s paired t-test. (B) Inverse relationship of Hif1a and Abca1 mRNA levels in PEC from pristane-treated mice. (C) PEC were collected 14 days after pristane- or MO-treatment and expression of HIF1α-regulated genes (Pfkl, G6pd, and Hk2) was measured (Q-PCR) (*P < 0.05, unpaired Welch’s t-test). (D) Adherent peritoneal cells from pristane-treated mice were incubated with GW3965 or DMSO for 24 h, and expression levels of Hif1a, Hk2, Pfkl, and Abca1 mRNA were measured relative to 18 S (Q-PCR). (*P < 0.05, **P < 0.01, unpaired Welch’s t-test).