FIG 2 .

Phenotypic characterization of pyruvate kinase mutants. (A) Wild-type and pyk1 and pyk2 transposon mutants of S. coelicolor grown on nutrient agar and minimal medium (MM) with glucose, with strains complemented with either pyk1 or pyk2 in trans and WT strains with additional copies of pyk1 or pyk2. (B) Growth curve of S. coelicolor WT, pyruvate kinase mutants, and complemented strains in liquid YEME medium. (C and D) Coelimycin production yield (absorption unit [AU] per milligram of biomass) (C) and undecylprodigiosin (RED) yield during growth in YEME medium (D). Symbols: black circles, WT; blue squares, pyk1 pyruvate kinase mutant; red inverted triangles, pyk2 pyruvate kinase mutant; purple triangles, pyk1-complemented strain; pink diamonds, pyk2-complemented strain.