TABLE 1 .
S. aureus strains used in this study
| Strain | Genotype | Description | Source or reference |
|---|---|---|---|
| Newman | WT | Wild-type, methicillin-sensitive clinical isolate | 57 |
| Newman | pbgS | pbgS (NWMN_1562) gene interrupted with erythromycin resistance gene ermB by homologous recombination, transduced into Newman | 3 |
| Newman | ΔchdC | In-frame unmarked deletion of chdC (NWMN_0550) generated by allelic exchange | 58 |
| Newman | ΔhemX ΔchdC | In-frame unmarked deletion of chdC (NWMN_0550) generated by allelic exchange in ΔhemX strain | This work |
| Newman | ΔmenB | In-frame unmarked deletion of menB generated by allelic exchange | 59 |
| Newman | ΔqoxB cydB | In-frame unmarked deletion of qoxB and cydB::Tn | 3 |
| JE2 | katA | katA::Tn (NE1366) | BEI (46) |
| Newman | katA | katA::Tn (NE1366), transduced into Newman | This work |
| Newman | ΔhemX | In-frame unmarked deletion of hemX (NWMN_1565) | This work |
| RN9011 | RN4220 carrying pRN7023 integrase plasmid | 24 | |
| RN9011 | attC::Plgt | pJC1111 Plgt integrated into chromosome at attC locus | This work |
| RN9011 | attC::PlgthemX | pJC1111 PlgthemX integrated into chromosome at attC locus | This work |
| Newman | ΔhemX attC::Plgt | pJC1111 Plgt integrated into chromosome at attC locus | This work |
| Newman | ΔhemX attC::PlgthemX | pJC1111 PlgthemX integrated into chromosome at attC locus | This work |
| Newman | attC::Plgt | pJC1111 Plgt integrated into chromosome at attC locus | This work |
| Newman | ΔhemX pbgS | In-frame unmarked deletion of hemX (NWMN_1565) in pbgS strain | This work |
| Newman | ΔhssRS | In-frame unmarked deletion of hssRS generated by allelic exchange | 60 |
| Newman | ΔhrtB | In-frame unmarked deletion of hrtB generated by allelic exchange | 61 |
| Newman | hrtB | hrtB::Tn(PhiNE01762) | 10 |
| Newman | ΔhemX hrtB | hrtB::Tn(PhiNE01762) allele transduced to ΔhemX strain | This work |
| JE2 | cysG | cysG::Tn (NE1931; SAUSA300_2553::Tn) | BEI (46) |
| Newman | cysG | cysG::Tn (NE1931) transduced into Newman | This work |
| Newman | ΔhemX cysG | cysG::Tn (NE1931) transduced into Newman ΔhemX | This work |
| JE2 | nirD | nirD::Tn (NE1279) | BEI (46) |
| Newman | nirD | nirD::Tn (NE1279) transduced into Newman | This work |
| Newman | ΔhemX nirD | nirD::Tn (NE1279) transduced into Newman ΔhemX | This work |
| Newman | ΔgtrR-hemX | In-frame unmarked deletion of gtrR and hemX (NWMN_1565-1566) generated by single allelic exchange | This work |
| RN4220 | Restriction-deficient cloning intermediate strain | 62 |