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Structured illumination microscopy images of C. crescentus cells attached by their holdfast. Cells were stained using HADA (to label peptidoglycan), while NAG and DNA residues in the holdfast were labeled using AF-594 WGA (red) and YOYO-1 (green), respectively. Representative Z-stack images are presented. For the holdfast zoom panels, the average intensities for the entire stack for the green and red channels are projected together and merged in a single image.
