FIG 5 .

Viral DNA and transcript levels during acute and latent infection in mice infected with ΔCTRL2 or CTRL2R virus. TGs from infected mice were harvested at 7 dpi, 30 dpi, or following explant reactivation, and total RNA and DNA were isolated and quantified. (A) Viral genomes were measured by qPCR and normalized to the cellular DNA control. (B) Viral RNA transcript levels for LAT were measured at 7 and 30 dpi and following explant reactivation. (C to I) The miRNAs miR-H2 (C), miR-H4 (D), miR-H6 (E), and lytic transcripts ICP0 (F), ICP27 (G), tk (H), and gC (I) were measured by quantitative reverse transcription-PCR with specific primers and normalized to a cellular control and viral genome copy number. Statistical significance was evaluated with the Mann-Whitney test.