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. 2015 Sep;185(9):2364–2378. doi: 10.1016/j.ajpath.2015.05.016

Figure 5.

Figure 5

Postnatal day 20 (P20) immunofluorescence analysis of survivin and YAP-isolated SVZ (A–J), CC (K–O), and cortical fiber (P–T) tissues harvested from normoxia-reared nursing pups treated with either plain water, 5% sucrose (vehicle), or minocycline, and reduced oxygen–reared nursing pups treated with either plain water, 5% sucrose (vehicle), or minocycline at P20. A–J: Immunofluorescence analyses of P20 NX H2O, NX SC, NX SC+MC, HX SC, and HX SC+MC pups reveal increased endothelial survivin (A–F) and YAP (F–J) expression as evidenced by increased survivin and CD31 colocalization and YAP and CD31 colocalization in reduced oxygen, minocycline-treated pups compared to NX H2O, NX SC, and HX SC+MC pups. White arrows in J, inset, denote YAP–CD31 endothelial cell colocalization. K–T: Immunofluorescence analyses of P20 NX H2O, NX SC, NX SC+MC, HX SC, and HX SC+MC pups reveals increased PLP expression in the CC (K–O) and cortical fiber tracks (P–T) in reduced oxygen minocycline-treated pups compared to NX H2O, NX SC, and hypoxic vehicle-treated pups. Pups reared in normoxic conditions treated with minocycline exhibit increased PLP expression compared to NX H2O–treated, and NX SC–treated pups. n = 3. Scale bars: 100 μm. CC, corpus callosum; HX SC, reduced oxygen, vehicle-treated; HX SC+MC, minocycline-treated; LV, lateral ventricle; NX H2O, normoxic water-treated; NX SC, vehicle (5% sucrose)-treated; NX SC+MC, minocycline-treated; PLP, proteolipid protein; SVZ, subventricular zone; YAP, yes-associated protein 1.