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. 2018 Jan 8;10(2):188–199. doi: 10.15252/emmm.201708292

Figure EV1. RASGRP1 expression is not detected in T‐cell blasts with the anti‐RASGRP1 antibodies PA5‐25750 and EPR9609.

Figure EV1

Comparison of RASGRP1 expression in T‐cell blasts of a healthy control (Ctr.) and P1.1 (Pat.) and in HEK293T cells transfected with empty vector, WT‐RASGRP1 or RASGRP1A638GfsX16.
  1. RASGRP1 detection using the anti‐RASGRP1 antibody PA5‐25750. Actin was used as a loading control. Detection of RASGRP1 required long exposure (1 h to overnight).
  2. RASGRP1 detection using the anti‐RASGRP1 antibody EPR9609. Actin was used as a loading control. Detection of RASGRP1 required long exposure (1 h to overnight).
Data information: One representative of three independent experiments.Source data are available online for this figure.