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. 2018 Jan 8;10(2):188–199. doi: 10.15252/emmm.201708292

Figure 2. Defective ERK1/2 phosphorylation but normal tyrosine phosphorylation signals and calcium flux in activated RASGRP1‐deficient T cells.

Figure 2

  1. Immunoblot showing the global tyrosine phosphorylation in T‐cell blasts from a control donor (Ctr.) and P1.1 (Pat.) stimulated with anti‐CD3 antibodies for 0, 5, 10, 15, and 30 min. One representative of two independent experiments from different blood samples.
  2. Flow cytometry analyses of Ca2+‐flux in T‐cell blasts of a control donor and P1.1 loaded with the Ca2+‐sensitive fluorescent dye Indo‐1. Cells were then stimulated with anti‐CD3 antibody (first arrow) crosslinked with rabbit anti‐mouse antibody (second arrow) and then incubated with ionomycin. Intracellular Ca2+ levels are expressed in arbitrary units (A.U).
  3. Immunoblots showing phosphorylation of PLCG1 (P‐PLCG1), ERK (P‐ERK 1/2), p38 (P‐p38), and AKT (P‐AKT) in T‐cell blasts from a control donor and P1.1 stimulated with anti‐CD3 antibody for 0, 5, 10, 15, and 30 min. Total ERK 1/2, RASGRP1, and actin (as loading control) are also shown. One representative of three independent experiments from different blood samples.

Source data are available online for this figure.