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. 2018 Jan 8;10(2):188–199. doi: 10.15252/emmm.201708292

Figure 5. RASGRP1 is required for T‐cell expansion in response to CD3‐TCR activation.

Figure 5

  1. Rescue of T‐cell proliferation by expression of wild‐type RASGRP1 in RASGRP1‐deficient T cells. Immunoblots for RASGRP1 and actin as a loading control of control (Ctr.) and RASGRP1‐deficient T cells of patient P1.1 (Pat.) transduced by empty or wild‐type RASGRP1‐containing vector with mCherry gene reporter at day 14 of culture.
  2. Proliferation of control (Ctr.) and RASGRP1‐deficient T cells of patient P1.1 (Pat.) transduced by empty or wild‐type RASGRP1‐containing vector. Representative histograms of CellTrace violet dye dilution showing the cell divisions of mCherry‐positive (mCherry+) and mCherry‐negative (mCherry−) cells after stimulation with two concentrations of anti‐CD3 antibody, with histograms in gray corresponding to unstimulated cells (unstim.).
  3. Curves of the percentage of mCherry‐positive transduced cells after repeated stimulations indicated by gray arrows with anti‐CD3 (upper panels) or anti‐CD3/CD28 (lower panels) antibodies at different days in long‐term expansions.
Data information: Representative data from two independent experiments.Source data are available online for this figure.