Figure 6. CTPS1 upregulation is impaired in activated RASGRP1‐deficient T cells.

• A
  Immunoblots for CTPS1 expression of control donor (Ctr.) and P1.1 T‐cell blasts (Pat.) stimulated with anti‐CD3/CD28 antibodies for different periods of time. Ku70 was used as a loading control. One representative of two independent experiments from different blood samples.
• B
  Histograms of intracellular staining for CTPS1 expression in T cells from a control donor in which RASGRP1 expression was silenced with plasmids containing shRNA for RASGRP1 (Sh RASGRP1) or scramble shRNA (Sh scramble) with GFP gene reporter. Cells were stimulated with anti‐CD3/CD28 antibodies or not (non stim.) for 48 h. One representative of two independent experiments from blood samples of two different healthy donors.
• C, D
  Control donor T‐cell blasts stimulated or not (non stim.) with anti‐CD3/CD28 beads in the presence or not of 40 μM of deazauridine. In panel (C), proliferation was analyzed at day 3 of stimulation. In panel (D), CTPS1 expression was analyzed at 48 h of stimulation. Actin as a loading control.

Source data are available online for this figure.