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. 2017 Nov 17;191(3):255–267. doi: 10.1111/cei.13075

Figure 3.

Figure 3

Inflammation of orbital tissue from mouse thyroid stimulating hormone receptor (TSHR) A‐subunit‐immunized mice evaluated by magnetic resonance imaging (MRI) and F4/80 staining. Immune animals 6 weeks after the last immunization; five mice from each group were selected randomly for MRI. At least 48 h before imaging, animals were injected intravenously (i.v.) with perfluorcarbon (PFC), which is taken up avidly by circulating monocytes and macrophages and inflammatory tissue detected with 19F M RI. (a) Quantification of 19F signal from MRI expressed as 19F integral in arbitrary units (a.u.). Significantly increased orbital inflammation in mouse TSHR A‐subunit immunized animals compared to controls, *P < 0.05. B: F4/80 positive cells were counted in the periorbital tissue (in No/mm2). Significant increased localized inflammation in animals undergoing experimental GO in comparison to control β‐Gal was found in muscle and perineural tissue (****P < 0.001). (c) CD3‐positive cells were counted in the periorbital tissue (in No/mm2). Again, most of the CD3‐positive cells in mouse TSHR‐immunized mice were localized in muscle and periorbital tissue in comparison to control β‐Gal orbits. (d,e) Section in the middle region of the orbital perineural region from mouse TSHR A‐subunit immune animal (×400). The optic nerve is marked as ‘nerve’ in the photomicrographs, adipose tissue as ‘AT’. The arrows in photomicrographs indicate areas of F4/80 active accumulation of inflammatory cells in the perineural tissue (d) and muscle tissue (e) of animals undergoing experimental GO. (f,g) Section in the middle region of orbital muscle from mouse TSHR A‐subunit immune animal undergoing CD3 IHC (×400). The arrows in photomicrographs indicate areas of CD3‐positive cell accumulation in the perineural tissue (f) and in the orbital muscle (g) of animals undergoing experimental GO. Overall, the MRI studies and immunohistochemistry (IHC) studies both provide independent evidence for increased inflammation in the orbital tissue of animals undergoing experimental GO. [Colour figure can be viewed at wileyonlinelibrary.com]