Skip to main content
. 2018 Mar;93(3):208–215. doi: 10.1124/mol.117.110445

Fig. 5.

Fig. 5.

Effects of FCCP, EIPA, ouabain, or monensin on net MTX levels in RFC stable transfectants (A) and transient transfectants of RFC or PCFT (B). (A) Net uptake of MTX (5 μM) in R1-11-RFC-6 cells incubated in HBS at pH 7.40 or RPMI at pH 7.3 for 40 minutes. (B) Net uptake of MTX in RFC (5 μM)- or PCFT (100 μM)-transient transfectants in HBS at pH 7.40 for 40 minutes. FCCP (10 μM), EIPA (10 μM), ouabain (10 μM), or monensin (50 μM) was added simultaneously with [3H]MTX. For (B), the data are expressed as percentages of control, which is 11 ± 2 and 300 ± 52 (mean ± S.D.) pmol/mg protein for RFC and PCFT transient transfectants, respectively. Asterisks indicate that the values are significantly different from the control on the basis of a paired two-tailed t test (P < 0.5). Data are the mean ± S.D. from three (A) and four (B) independent experiments.