Fig. 4.

3D search query of two commercially available databases, the Chembridge and the Maybridge databases, which together have over a million chemicals, resulted in a refined hit list of 167 compounds. (A) Represents a schematic of the 3D virtual screening of the ChemBridge and Maybridge databases. (B) Dose-response data of BRET assays in HEK293 cells, illustrating dimerization profile of selected hits. Data are shown as mean ± S.D. of three biologic replicates. Data are normalized to DMSO control. (C) Measurement of compound binding to ERα and ERβ using in vitro fluorescence polarization competition binding assays.