Table 2. Intra-assay and inter-assay accuracy and precision of metronidazole (MET), ceftriaxone (CEF), and hydroxymetronidazole (MET-OH) in plasma, and CEF in ultra-filtrate (CEF ^uf) at LLOQ, LOQ, MOQ and HOQ.

Intra-assay (n=6)	Compound	Nominal concentration (µg/ mL)	Mean estimated concentration (µg/ mL) ±SD	Precision (CV %)	Accuracy (%)
	MET	0.05	0.051 ± 2.0	3.9	101.9
		0.15	0.148 ± 7.7	7.8	98.7
		20	20.44 ± 4.0	3.9	102.2
		40	37.75 ± 5.6	5.9	94.4
	CEF	0.4	0.39 ± 2.5	3.2	97.5
		1.2	1.10 ± 1.5	1.7	91.7
		120	112.02 ± 3.5	3.7	93.3
		240	219.51 ± 6.8	7.5	91.5
	MET-OH	0.02	0.018 ± 1.7	2.6	90.0
		0.06	0.057 ± 4.7	4.9	95.0
		12	11.43 ± 2.5	2.7	95.2
		24	24.49 ± 8.6	8.4	102.0
	CEF uf	0.4	0.41 ± 5.5	5.3	100.9
		1.2	1.27 ± 5.5	5.2	105.8
		120	112.32 ± 5.1	5.5	93.6
		240	253.03 ± 8.2	7.8	105.4
Inter-assay (n=18)	MET	0.05	0.051 ± 1.4	2.7	101.1
		0.15	0.155 ± 5.6	5.4	103.3
		20	20.59 ± 3.3	3.2	103.0
		40	38.79 ± 5.6	5.8	97.0
	CEF	0.4	0.40 ± 2.7	2.9	100.0
		1.2	1.15 ± 3.8	3.9	95.8
		120	114.54 ± 5.1	5.4	95.4
		240	226.75 ± 5.2	5.5	94.5
	MET-OH	0.02	0.019 ± 1.2	2.3	95.0
		0.06	0.059 ± 5.2	5.3	98.3
		12	12.01 ± 4.8	4.8	100.1
		24	24.51 ± 4.7	4.6	102.1
	CEF uf	0.4	0.43 ± 5.8	7.4	107.2
		1.2	1.22 ± 8.3	8.1	101.6
		120	115.32 ± 5.1	5.3	96.1
		240	251.30 ± 6.2	5.9	104.6