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. 2018 Feb 2;12:3. doi: 10.3389/fnint.2018.00003

Figure 2.

Figure 2

Identification of four types of PNNs. (A,B) Triple staining of WFA (red), PV (blue) and Cat315 (green in A) or Cat316 (green in B). Arrowheads and arrows in (A) indicate WFA+/Cat315+ and WFA−/Cat315+ PNNs, respectively. Arrows in (B) indicate WFA+/Cat316+ PNNs, Scale bar, 50 μm. (C) Four types of PNNs, which differ in the glycan structures of aggrecan. (D) Percentage of WFA+/Cat315+ (closed bar), WFA+/Cat316+ (gray and Cat315+ or Cat316+/WFA+ (open bar) PNNs (n = 131, 80 and 327 cells, respectively). (E) Fluorescence intensity of WFA-staining (left panels) and PV-staining (right panels) in Cat315+ PNNs (closed bar) relative to Cat315− PNNs (closed bar). n = 62 and 52 cells from three mice for Cat315− and Cat315+ PNNs, respectively. (F) Fluorescence intensity of WFA-staining and PV-staining in Cat316+ PNNs (closed bar) relative to Cat316− PNNs (closed bar). n = 72 and 55 cells from three mice for Cat316− and Cat316+ PNNs, respectively. Asterisks denote significant differences (P < 0.00001, Student’s t-test) between two groups. Error bars represent SEM.