Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 2;4:97. doi: 10.3389/fmolb.2017.00097

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Adinolfi, Puglisi, Crack, Iannuzzi, Dal Piaz, Konarev, Svergun, Martin, Le Brun and Pastore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cross-linking experiments to identify the interacting sites on IscX and IscS using different relative concentrations of IscS and IscX. (A) Experiments using wild-type IscS (8 μM) and IscX at molar ratios 1:10, 1:7.5, 1:5, 1:2.5, and 1:1 from left to right. The last two lanes on the right are the controls carried out using isolated IscS and IscX. As expected the IscS dimer disassembles in SDS and runs as a monomer of ca. 45 kDa. (B) The same as in A but with the IscS_R220/223/225E mutant. (C) The same as in A but with the IscS_K101/105E mutant. The markers are indicated on the right.