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. 2018 Feb 2;4:97. doi: 10.3389/fmolb.2017.00097

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Copyright © 2018 Adinolfi, Puglisi, Crack, Iannuzzi, Dal Piaz, Konarev, Svergun, Martin, Le Brun and Pastore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Iron dependence of the relative effect of CyaY and IscX on cluster assembly enzymatic kinetics on IscU. (A) Initial rates of kinetics of cluster formation on IscU as a function of iron concentration in the presence of IscU only (gray circle), IscU and CyaY (black rectangles) and IscU, IscX, and CyaY (gray triangles). (B) The same as in (A) but expanded for clarity. (C) Assays carried out in the absence and in the presence of IscX (1 μM) at increasing concentrations of iron (10 μM, 25 μM, 50 μM, 80 μM) to assess whether IscX is affected by iron. No CyaY was added to the assay. All experiment contained 1 μM IscS, 50 μM IscU, 250 μM Cys, 2 mM DTT.