Table 2.

Species-specific primers for the quantification of selected rumen bacterial populations using a real-time qPCR assay

Target bacterial species		Primer sequence (5' → 3')	Reference
Anaerovibrio lipolytica	F:^a	GAAATGGATTCTAGTGGCAAACG	[12]
Anaerovibrio lipolytica	R:^b	ACATCGGTCATGCGACCAA	[12]
Butyrivibrio proteoclasticus	F:	GGGCTTGCTTTGGAAACTGTT	[12]
Butyrivibrio proteoclasticus	R:	CCCACCGATGTTCCTCCTAA	[12]
Eubacterium ruminantium	F:	CTCCCGAGACTGAGGAAGCTTG	[37]
Eubacterium ruminantium	R:	GTCCATCTCACACCACCGGA	[37]
Fibrobacter succinogenes	F:	GCGGGTAGCAAACAGGATTAGA	[37]
Fibrobacter succinogenes	R:	CCCCCGGACACCCAGTAT	[37]
Megaspheara elsdenii	F:	AGATGGGGACAACAGCTGGA	[37]
Megaspheara elsdenii	R:	CGAAAGCTCCGAAGAGCCT	[37]
Prevotella bryantii	F:	AGCGCAGGCCGTTTGG	[37]
Prevotella bryantii	R:	GCTTCCTGTGCACTCAAGTCTGAC	[37]
Selenomonas ruminantium	F:	CAATAAGCATTCCGCCTGGG	[37]
Selenomonas ruminantium	R:	TTCACTCAATGTCAAGCCCTGG	[37]
Succinimonas amylolytica	F:	CGTTGGGCGGTCATTTGAAAC	[29]
Succinimonas amylolytica	R:	CCTGAGCGTCAGTTACTATCCAGA	[29]
Streptococcus bovis	F:	TTCCTAGAGATAGGAAGTTTCTTCGG	[37]
Streptococcus bovis	R:	ATGATGGCAACTAACAATAGGGGT	[37]
Succinivibrio dextrinosolvens	F:	TAGGAGCTTGTGCGATAGTATGG	[29]
Succinivibrio dextrinosolvens	R:	CTCACTATGTCAAGGTCAGGTAAGG	[29]
Bacteria general 1	F:	GGATTAGATACCCTGGTAGT	[20]
Bacteria general 1	R:	CACGACACGAGCTGACG	[20]
Bacteria general 2	F:	GTGSTGCAYGGYTGTCGTCA	[19]
Bacteria general 2	R:	ACGTCRTCCMCACCTTCCTC	[19]

^aF forward primer; ^bR reverse primer