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High-performance thin-layer chromatography (HPTLC) analysis of diverse sphingolipid species in MCF-7 cells. Representative HPTLC plate showing the separation of ceramides, DAG and non-complex GSLs like GlcCer, LacCer and complex GLSs like Gb3 and gangliosides. MCF-7/naiv: untransfected, MCF-7/pTarget: empty vector-transfected, MCF-7/NHT: CRISPR-Cas non-human target control, MCF-7/UGCG OE: UGCG expression plasmid-transfected, MCF-7/KD: CRISPR-Cas UGCG Knockdown. M = Marker
