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Production of TNF-α (upper) and IL-6 (bottom) by adherent mouse peritoneal macrophages stimulated with S. aureus HU-85a (agr+) or HU-85 (agr-deficient). Each bar represents cytokine concentrations obtained in representative experiments performed in triplicate. Comparisons of cytokine concentrations produced by stimulation with the wild-type compared with stimulation with the agr-deficient derivative were analyzed using the Student t-test.
