KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bacterial and Virus Strains | ||
| BL21 Star DE3 | Life Technologies | C601003 |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| AlexaFluor 488 maleimide | ThermoFisher | A10254 |
| AlexaFluor 555 NHS ester | ThermoFisher | A37571 |
| hnRNPA2 LC (190-341) hnRNPA2 LC D290V hnRNPA2 LC P298L hnRNPA2 LC WT S285C hnRNPA2 LC WT S329C hnRNPA2 LC D290V S285C hnRNPA2 LC D290V S329C hnRNPA2 LC P298L S285C hnRNPA2 LC P298L S329C MBP-hnRNPA2 full length MBP-hnRNPA2 full length D290V MBP-hnRNPA2 full length P298L MBP-hnRNPA2 ΔLC (i.e. hnRNPA2 1-189) MBP-hnRNPA2 LC (190-341) MBP-hnRNPA2 LC (190-341) R191K R254K MBP-hnRNPA2 LC (190-341) R191K R201K R216K R254K |
This study | n/a |
| TDP-43 CTD | This study/Conicella et al | n/a |
| PRMT1 | AtGen | PRM0802 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| hnRNPA2 LC WT | This study | BMRB: 27123 |
| hnNRPA2 LC D290V | This study | BMRB: 27124 |
| hnRNPA2 LC P298L | This study | BMRB: 27299 |
| TDP-43 CTD | Conicella et al. | BMRB: 26823 |
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| Recombinant DNA | ||
| hnRNPA2_LC_WT | This study | Addgene: 98657 |
| hnRNPA2_LC_D290V | This study | Addgene: 98658 |
| hnRNPA2_LC_P298L | This study | Addgene: 104465 |
| hnRNPA2_LC_D220V | This study | Addgene: 98659 |
| hnRNPA2_LC_D230V | This study | Addgene: 98660 |
| hnRNPA2_LC_WT_S285C | This study | Addgene: 98665 |
| hnRNPA2_LC_WT_S329C | This study | Addgene: 98667 |
| hnRNPA2_LC_D290V_S285C | This study | Addgene: 98666 |
| hnRNPA2_LC_D290V_S329C | This study | Addgene: 98668 |
| hnRNPA2_LC_P298L_S285C | This study | Addgene: 104469 |
| hnRNPA2_LC_P298L_S329C | This study | Addgene: 104470 |
| MBP-hnRNPA2_LC | This study | Addgene: 98661 |
| MBP-hnRNPA2_LC_R191K_R254K | This study | Addgene: 104466 |
| MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K | This study | Addgene: 104467 |
| MBP-hnRNPA2_FL_WT | This study | Addgene: 98662 |
| MBP-hnRNPA2_FL_D290V | This study | Addgene: 98663 |
| MBP-hnRNPA2_FL_P298L | This study | Addgene: 104468 |
| MBP-hnRNPA2_ΔLC | This study | Addgene: 98664 |
| TDP-43 CTD | Conicella et al. 2016 | Addgene: 98669 |
| Software and Algorithms | ||
| nmrPipe | Frank Delagio | https://www.ibbr.umd.edu/nmrpipe/install.html |
| ImageJ | NIH | https://imagej.nih.gov/ij/download.html |
| SSP | Julie Forman-Kay | http://abragam.med.utoronto.ca/software.html |
| Delta2D | Michele Vendruscolo | http://www-mvsoftware.ch.cam.ac.uk/ |
| GROMACS-4.6.7 | Lindahl et al., 2001 | |
| HOOMD-Blue v2.1.5 | Anderson et al., 2008 | |
| Plumed 2.1 | Tribello et al., 2014 | |
| Other | ||