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. 2018 Feb 6;11:83. doi: 10.1186/s13071-018-2664-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — SEM analysis of T. cruzi during metacyclogenesis. a Control epimastigote not subjected to nutritional stress. The flagellum emerges from the anterior portion of the cell body (arrow). b Protozoan subjected to nutritional stress for 2 h. c Adhered cells after 48 h in differentiation medium. The flagellum emerges from the middle of the cell body (arrow). d Cells collected from the supernatant after 72 h in differentiation medium. Arrowheads indicate membrane shedding at the flagellar pocket. e, f Purified trypomastigotes fraction. e Protozoan displaying an epimastigote-like form with the flagellum emerging from the posterior end of the cell body (arrow). f A parasite displaying a typical metacyclic trypomastigote morphology. Arrowheads indicate membrane shedding. Scale-bars: a, 5.0 μm; b-f, 2.5 μm