. 2017 Apr 30;4:119–126. doi: 10.1016/j.biopen.2017.04.002

Table 2.

Using differentiating and non-differentiating cell lines for RT-PCR analysis of genes that showed significantly higher expression levels in colon epithelium compared to fibroblasts.^a

Genes	Array expression^b (Unnormalized signal)		Expression level determined by RT-PCR^c (Normalized by the expression level of Gapdh)
	MEF^b (n = 6)	Colon^b (n = 9)	MDCK cells^c (n = 3 each day)			IEC-6 cells^c (n = 3 each day)			C2C12 cells^c (n = 3 each day)
	MEF^b (n = 6)	Colon^b (n = 9)	Day 2	Day 13	Day 17	Day 2	Day 13	Day 17	Day 2	Day 13	Day 17
MYLK	3380	45531	4.10	2.90	2.19
MYO7B	47	7709	0.35^A	1.84^B	5.47^C	0.23^A	1.23^B	1.24^B	0.10^A	0.45^B	1.14^C
SDCBP2	396	64199	ND^A	5.53^B	1.89^A	ND	ND	ND	0.63	0.60	0.54

KIF5A	623	2578	2.42^A	6.98^B	6.43^B	0.68^A	0.98^B	1.06^B	0.66^A	0.74^A	1.22^B
KIF12	18	1785	3.66^A	6.78^B	6.27^B	1.12	0.93	0.95	ND	ND	ND
KIF13A	208	548	2.75^A	7.49^B	6.77^B	1.23	1.10	1.10	0.17^A	0.98^B	0.85^B
KIF21A	231	790	3.74^A	5.97^B	3.54^A
KIF27	9	154	1.51^A	5.53^B	5.26^B	ND	ND	ND	ND	ND	ND

RAB15	4250	54009	5.60	7.13	6.06
RAB19	171	5795	5.10	5.12	4.42
RAB25	23	13342	6.93	6.50	4.98
RAB27A	260	1981	7.48^A	5.14^B	2.91^C	1.40^A	0.69^B	0.88^B	0.83^A	0.42^B	0.67^C
RAB27B	86	2781	1.42^A	4.70^B	3.20^C	1.17^A	0.89^B	0.79^B	0.49^A	1.02^B	0.66^C

^A,B,CDifferent superscript capital letters indicate significantly different from each other within the same cell type at p < 0.01. ND: not detectable.

Data of gene expression array and RT-PCR analysis shown in this table are means of groups. Three genes with expression levels in bold fonts meet all proposed criteria as candidate machinery genes in epithelial apical and basolateral protein targeting.

These gene expression microarrays used cDNA prepared from mouse embryonic fibroblasts (MEF) or from colon epithelium (Colon). The differences between two groups are significant at p < 0.01.

These gene expression analyses used cDNA of MDCK cells (canine kidney epithelium-origin) at Day 2 after seeding (prior to differentiation) and Day 13 and 17 after seeding (differentiated); IEC-6 cells (rat intestinal epithelium-origin but non-differentiating) after the same interval of culturing as other two cell lines; C2C12 cells (mouse skeletal muscle-origin) at Day 2 after seeding (prior to differentiation) and Day 13 and 17 after seeding (differentiated).