Fig. 1.

A. Representative Pro-Q Diamond phospho-protein stained SDS-Polyacrylamide (Tris-Glycine) gel image showing phosphorylation of Bax protein (GST tagged) by JNK3 enzyme. Lane 1: Phosphoprotein standard; Lane 2: Negative Control, i.e. Bax protein + Mg²⁺ATP; Lane 3: Experimental Sample, i.e. Bax protein + JNK3 + Mg²⁺ATP; Lane 4: Positive Control, i.e. Bax protein + JNK3 + Mg²⁺ATP + Calf Intestinal Alkaline Phosphatase. B. Coomassie blue dye stained image of the same gel, no bands could be seen in Lane 1 because of the low concentration of proteins in the phosphoprotein standard. Mw marker: Color Burst electrophoresis Marker C1992 (Mol. wt. 8000 ─ 220,000 Da, Sigma Chem. Company, USA). The size of the gel increases when stained with Pro-Q Diamond dye and then decreases to the original size during coomassie blue staining.