Fig. 3.

Current–Voltage relation (I–V plot) of the full open state of the Phosphorylated (by JNK3) Bax protein pore. Pore state showing the maximum current value in the single-channel current–time traces was considered as full open state of the Phospho-Bax pore. Full open state current values were obtained from the current–time traces and their current amplitude histograms at different voltages. Finally, mean of the current values of the full open state obtained from three independent sets of experiments was shown by the I–V plot. I–V plot was obtained using pClamp 10.2 (Axon Instruments, Sunnyvale, CA, USA) and Origin 5.0 (Origin Lab Corp., MA, USA) software. I–V plot of the Phospho-Bax pore was fitted with a linear function. Best fit analysis of the experimental data was done using Origin 5.0. Values are Mean ± S.E. of three independent sets of experiments.