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. 2017 Dec 19;39(3):1043–1051. doi: 10.3892/or.2017.6166

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Copyright: © Quan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Expression changes in osteoclast markers detected by real-time PCR and western blotting. (A) Indirect cell co-culture model, SCC25 cells were added to the top of the Transwell insert, while CD14⁺ monocytes were plated to the bottom of 24-well plates. (B) For monocytes without stimulation from CM of SCC25 cells, NFATc1 was expressed on day 3 and until day 6; proteinases MMP-9 and CTSK were increased on days 3 and 6. Similar expression trends in NFATc1 were observed in monocytes after stimulation with SCC25 CM. For MMP-9 and CTSK, SCC25 CM stimulated their expression from the start of co-culture, which reached a maximum on day 6; (C and D) Protein level changes in NFATc1, MMP and CTSK were further confirmed by western blotting.