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PF inhibits Skp2 expression at mRNA and protein levels. (A) U87 and U251 glioma cells were treated with indicated concentrations of PF and Skp2 gene expression was analyzed by quantitative real-time PCR. (B) Effect of PF on Skp2, P21, P27 and p-AKT protein levels, as determined by immunoblotting. (C) Quantitative analysis of results showed in (B). *P<0.05, **P<0.01 vs. control.
