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. 2017 Dec 28;39(3):1052–1062. doi: 10.3892/or.2017.6175

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Copyright: © Ouyang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of Skp2 overexpression on glioma cell growth, apoptosis and invasion. (A) Effect of PF in combination with Skp2 cDNA transfection on glioma cell growth was evaluated with CCK-8. Control, pcDNA3.1. cDNA, Skp2 cDNA; Both, Skp2 cDNA+ PF. *P<0.05 vs. control, ^#P<0.05 vs. either PF treatment or Skp2 cDNA transfection alone. (B) Left panel, apoptosis of U87 glioma cells, as determined by flow cytometry following Skp2 cDNA transfection and PF treatment. Right panel, quantitative analysis of results shown in the left panel. (C) Cell invasion was evaluated with the Transwell assay in cells overexpressing Skp2 and treated with PF. (D) Quantitative analysis of results shown in (C). *P<0.05 vs. control.