Fig. 2.

Best representative single-channel current–time traces chosen from three different sets of experiments (and their histograms) recorded with respect to the ground on a DPhPE/DPhPC membrane with a symmetrical bath solution of 1 M KCl, 10 mM MgCl₂, 10 mM HEPES, pH 7.4 at 25 °C. A. Native VDAC at +10 mV showing its characteristic open-state (O) (O = 40 pA) which is represented by one peak fitted with Gaussian function in the histogram; B. Native VDAC at −10 mV showing the characteristic open-state (O) (O = −40 pA) which is represented by one peak fitted with Gaussian function in the histogram; C. Phosphorylated (by JNK3) VDAC at +10 mV showing the open P-state (P current = 32 pA) and frequent flickering to four substates (S1, S2, S3 and S4) which are seen in the histogram; D. Phosphorylated (by JNK3) VDAC at −10 mV showing the open P-state (P current = −32 pA) which is represented by one peak fitted with Gaussian function in the histogram. In all the traces the baseline is at 0 pA and it represents the fully closed state of the channel.