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Expression levels of ATF4 change following alteration of MYC expression. HUVECs and MG-63 cells were transfected with (A) MYC, ATF4 or MYC+shATF4-1 and (B) shMYC, shATF4 or shMYC+ATF4. (C and D) Cell death in the treatment groups was assessed after 24 h under attached or floating conditions. *P<0.05 vs. control. NS, no significance; sh, short hairpin; ATF4, activating transcription factor 4; MYC, myelocytomatosis oncogene.
