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. 2017 Dec 18;17(3):3658–3666. doi: 10.3892/mmr.2017.8296

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Copyright: © Mo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — MYC binds to the promoter of ATF4 to regulate its expression. (A) A total of 7 regions were designed of ChIP. (B and C) ChIP analysis demonstrated the association of MYC in transfected HUVEC and CHON-001 cells in region 5. (D) Luciferase reporter assays were performed with the −1,332 to −626 segment-containing region where MYC regulates ATF4. ATF4, activating transcription factor 4; MYC, myelocytomatosis oncogene; ChIP, chromatin immunoprecipitation; TSS, transcription start site..